DNA

Part:BBa_K1778000:Design

Designed by: Lehua Jia   Group: iGEM15_Scut-Champion-Park   (2015-09-10)


CYC1TATA sequence is derived from the TATA box secific sequence of yeast Saccharomyces cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the use of the part in Pichia pastoris, we need to consider whether the sequence is able to achieve the desired purpose.Fortunately, we have found evidence in the literature that this is a key part of the Tet-on system in yeast.


Source

The sequence is from the plasmid pCM188, and the source of the original source is Saccharomyces cerevisiae.

References